Confocal Laser-Induced Fluorescence Detection System for a Microfluidic Chip Based on an Embedded System
X-B. Yang, W-P. Yan, X-F. Wang and N. Xue
To meet the demands of miniaturization and integration in the microfluidic detection system, an on bench confocal laser-induced fluorescence detection (LIF-D) system was designed to achieve the functions of image observation, fluorescence signal acquisition and auto focusing based on embedded Linux operating system and ARM9 microprocessor. Detection curves and images during the process of sample injection and separation could be visually displayed on the screen of a 3.5 inch liquid crystal display (LCD). The electrophoretic separation experiments with different concentrations of Rhodamine B samples were successfully performed in the microfluidic chip. The detection resolution of system was 1.0 × 10-7 mol/l. Furthermore, sample injection and separation were observed in the cross-channel area of microfluidic chip by the designed detection system, which would provide a useful tool to design and optimize the microfluidic chip and to track the sample separation process..
Keywords: Confocal laser-induced fluorescence detection, capillary electrophoresis microchip, ARM9 microprocessor, embedded Linux operating system