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Obtaining the Optical Properties of Chicken Stomach and Blood with Laser-Based Diffusewave Spectroscopy (DWS): An Ex Vivo Study
H. Ullah, E. A Khera and M. Malik

In this work we have investigated the light distribution and its correlation with the collected diffuse scattering for measurements of the optical properties (scattering coefficient and absorption coefficient) of normal, coagulated chicken stomach and chicken whole blood at the wavelengths of 630 and 870 nm using laser-based diffuse-wave spectroscopy (DWS). Our results reveal that the scattering and absorption coefficients of stomach vary with the change in wavelength; namely, at 870 nm the value of absorption coefficient was higher than at 633 nm for normal tissue while scattering coefficients shows the opposite trend than absorption coefficient in case of stomach. While in case of whole blood, we have explored that in the presences of the asymmetrical type of particles, the scattering coefficients decreases and absorption coefficient increases for all sucrose concentrations (0 to 400 mM); moreover, we concluded that the decrease in scattering for higher concentrations is due to refractive index mismatch effect between red blood cells and blood plasma. This technique provides the base for glucose levels detection and imaging of the deformations in microvasculature and superficial blood microvasculature by using optical coherence tomography technique both in vitro and in vivo.

Keywords: GaAs laser, He-Ne laser, chicken stomach, chicken blood, optical properties, blood rheology, Mie theory, diffuse wave spectroscopy (DWS)

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