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Measurement of Glucose and Cholesterol Concentration in Blood Using an Ultraviolet (UV)/Visible Spectrophotometer in the Wavelength Range 190 to 1100 nm
H. Ullah, A.R. Khan, Z. Batool, A. Nazir and R. Khawar

In this study we have investigated the potential association between blood glucose and total cholesterol level with complete blood count (CBC). We have calculated the contents of glucose and total cholesterol with help of spectrophotometric method (wavelength range of 190 to 1100 nm) related to absorbance values in test serum with enzymatic reagents. In our study, we have also related the mean value of total cholesterol and glucose level with CBC and reported how cholesterol and glucose level vary with the counting of blood cells in human blood. The correlation analysis indicated that there were strong correlations between glucose and total cholesterol level with CBC.

Keywords: Diode laser, laser beam, spectrophotometer, enzymatic method, blood glucose level, total cholesterol level, complete blood count (CBC)

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