Laser-assisted Multidimensional Imaging Modalities for Early Detection of Tumours in Bio Tissues: An In Vitro Study
M. Akhtar, H. Ullah, K. Aslam, M. A. Siddique, A. Akram, M. A. Majid, M. Abdullah-Al-Wadud, A. Aleem, M. Farhan and Z. Batool
The idea, upon which this research work depends, is the early detection of tumours in different biological soft tissues like bladder, respiratory and thigh tissues. Imaging and spectroscopic technique that were used to study healthy and cancerous tissues were white light microscopy and Fourier-transform infrared radiation (FTIR) spectroscopy. The observation and calculations collected through all these imaging techniques prove as helping tools to differentiate healthy and cancerous body tissues. Soft tissues from patients who had been diagnosed with bladder, respiratory, and thigh cancer were gathered from the Bahawalpur Victoria Hospital, Pakistan while keeping in mind the ethical research perspective. Two-dimensional (2-D) white light microscopy images showed that healthy and cancerous tissues have a different kind of shapes, sizes and structure arrangements. Normal tissues images show a regular pattern of structure arrangement, but cancerous tissues show an irregular shapes, sizes, and structures. Similarly, FTIR spectroscopy graphs represents different peaks of stretching vibrations of C-H, O-H, C-C, N-H, CH2 double and triple bonding of different biological tissues like bladder, respiratory and thigh tissues. This research work will help in the medical field for early detection of tumour which helps to diagnose the tumour and also helpful for better treatment planning in early stage.
Keywords: Diode laser, tumour, white light microscope, Fourier-transform infrared radiation (FTIR), Ewing sarcoma, genitourinary malignancies, microscopy, spectroscopy