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Fabrication of Elastic Substrates for Tissue Engineering by Two-Photon Initiated Polymerization
Noriko Kimura, Toshiyuki Watanabe, Kenro Totani, Akiko Oguni, Iichiro Harada and Toshiro Akaike

Elastometric substrates for tissue engineering was fabricated by using two-photon initiated polymerization. Ring shape patterns were obtained by using hydrophobic two-photon initiator, whereas hydrophilic two-photon initiator give needle-like posts after scanning laser light. Swiss 3T3 cells were cultivated on hydrogel micro-needle posts and the traction force between cells and extracellular matrix were measured. Effects of elastic modulus on cell function were discussed.

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