Studying the Biological Effect of Ultraviolet A Radiation on the Blood and Trace Elements Concentration in Rats Skin Tissue
M. Adel Fawzy, Mona Abdel-Hamed Yehia, Sameh M. El Ghnam, Basma A. Abdelmgeed and Y.H. Elbashar
Ultraviolet-A (UVA) radiation comprises more than 90% of the solar ultraviolet (UV) radiation reaching the earth. Exposure to UVA has been associated with dermatological disorders including skin cancer. Despite their preponderance amongst the UV range received on earth, the biological impacts of long wave UVA rays (340–400 nm) upon human skin have not been investigated so thoroughly. Nevertheless, recent studies have proven their harmful effects and involvement in carcinogenesis and immunosuppression. The aim of this study was to investigate the effect of the UVA radiation on the skin tissue of a living organism. Among this study the trace elements concentration using the Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-OES) technique, blood counts have been deduced. Forty male wistar albino rats (180-200) gm. are included in the study and divided into four groups, each containing ten rats. Group I was formed to be the control group, which was not exposed to UV radiation. Other groups were formed to observe the effects of UVA with different exposure doses on the skin. For all studied groups the trace elements and Complete blood counts after 17, 27 and 59 days exposure in continue form (24 hr. /dy) were examined. Statistical analyzed using IBM SPSS software package version 20.0 was used, (Armonk, NY: IBM Corp). The Kolmogorov-Smirnov test was used to verify the normality of distribution Quantitative data was described using mean and standard deviation. Significance of the obtained results was judged at the 5% level. All groups exposed to UVA radiation showed a statistically significant variation in trace elements concentration and complete blood counts. However, a comparison between the control groups and the different other groups, shows the effects of UV exposure on the skin and blood.
Keywords: ultraviolet –A radiation, Trace elements concentration, complete blood analysis, rats skin tissue.