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Properties of Biomaterials Obtained By Solid State or Submerged Biosynthesis
Nicoleta Radu and Ileana Rau

Biomaterials with therapeutic properties can be obtained by submerged biosynthesis with Monascus sp. This material is separated from culture media by filtration on cartridge with d<0.2 μm, and the filtrate which contained red biopigments are subjected to investigation related to stability of biomaterial at various pH and solvents for different periods of time. At the same time physical-chemical properties of this material are investigated through FT-IR and UV-VIS spectra. Results obtained revealed the good stability of biomaterial in the aqueous media and pH in the range 4-8, and the presence of N-glucosylrubropunctamine, N-glucosylmonascorubramine, N-glutarylrubropuntcamine and N-glutarylmonascoru- bramine in the biomaterial. The molar extinction coefficient (ε) varied function of dilution solvent (water, ethanol or butanol) and bathocromic and hyperchromic effects were observed, depending on concentration. The use of n-butanol as extraction solvent stabilized the pigment against the daylight for several weeks, promoting the stability of this type of complex. According to certain reports these pigments are reasonably stable in a wide range of pH but these pigments are unstable towards light.

Keywords: biopigment, solid state submerged biosynthesis.

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